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Combined NanoSIMS and Synchrotron X-ray fluorescence reveals distinct cellular and subcellular distribution patterns of trace elements in rice tissues

DOI: 10.1111/nph.12497 DOI Help
PMID: 24107000 PMID Help

Authors: Katie Moore (University of Oxford) , Yi Chen (Rothamsted Research) , Tina Geraki (Diamond Light Source) , Fred Mosselmans (Diamond Light Source) , Fangjie Zhao (Rothamsted Research)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: New Phytologist , VOL 201 (1) , PAGES 104-115

State: Published (Approved)
Published: September 2013
Diamond Proposal Number(s): 6042 , 7087

Abstract: The cellular and subcellular distributions of trace elements can provide important clues to understanding how the elements are transported and stored in plant cells, but mapping their distributions is a challenging task. The distributions of arsenic, iron, zinc, manganese and copper, as well as physiologically related macro-elements, were mapped in the node, internode and leaf sheath of rice (Oryza sativa) using synchrotron X-ray fluorescence (S-XRF) and high-resolution secondary ion mass spectrometry (NanoSIMS). Although copper and silicon generally showed cell wall localization, arsenic, iron and zinc were strongly localized in the vacuoles of specific cell types. Arsenic was highly localized in the companion cell vacuoles of the phloem in all vascular bundles, showing a strong co-localization with sulfur, consistent with As(III)–thiol complexation. Within the node, zinc was localized in the vacuoles of the parenchyma cell bridge bordering the enlarged and diffuse vascular bundles, whereas iron and manganese were localized in the fundamental parenchyma cells, with iron being strongly co-localized with phosphorus in the vacuoles. The highly heterogeneous and contrasting distribution patterns of these elements imply different transport activities and/or storage capacities among different cell types. Sequestration of arsenic in companion cell vacuoles may explain the limited phloem mobility of arsenite.

Journal Keywords: Arsenic; Trace Elements; Rice; Vascular Bundle; Nanosims; Synchrotron Xrf

Subject Areas: Environment, Biology and Bio-materials, Chemistry

Instruments: I18-Microfocus Spectroscopy