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Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1

DOI: 10.1107/S1744309113023828 DOI Help
PMID: 24100563 PMID Help

Authors: Stephen Carr (Institute of Molecular and Cellular Biology, University of Leeds) , Lauren B. Mecia (University of Leeds) , Simon Phillips (Diamond Light Source) , Christopher D. Thomas (University of Leeds)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology And Crystallization Communications , VOL 69 , PAGES 1123 - 1126

State: Published (Approved)
Published: October 2013

Open Access Open Access

Abstract: Antibiotic resistance in bacterial pathogens poses an ever-increasing risk to human health. In antibiotic-resistant strains of Staphylococcus aureus this resistance often resides in extra-chromosomal plasmids, such as those of the pT181 family, which replicate via a rolling-circle mechanism mediated by a plasmid-encoded replication initiation protein. Currently, there is no structural information available for the pT181-family Rep proteins. Here, the crystallization of a catalytically active fragment of a homologous replication initiation protein from the thermophile Geobacillus stearothermophilus responsible for the replication of plasmid pSTK1 is reported. Crystals of the RepSTK1 fragment diffracted to a resolution of 2.5 Å and belonged to space group P212121.

Journal Keywords: rolling-circle replication; plasmid pSTK1; plasmid pT181; replication initiation proteins; Staphylococcus aureus; Geobacillus stearothermophilus

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I04-1-Macromolecular Crystallography (fixed wavelength)