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Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis

DOI: 10.1073/pnas.1312313110 DOI Help
PMID: 24218553 PMID Help

Authors: Vijaykumar Karuppiah (University of Manchester) , R. F. Collins (University of Manchester) , A. Thistlethwaite (University of Manchester) , Ya Gao (The University of Manchester) , Jeremy Derrick (The University of Manchester)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Proceedings Of The National Academy Of Sciences

State: Published (Approved)
Published: November 2013

Abstract: Type IV pili are long, thin fibers, formed mainly of polymers of a single pilin protein, which are displayed on the surfaces of many bacteria, including several human pathogens. Here, we report three-dimensional reconstructions of the PilMNO inner membrane complex, alone and in complex with pilin protein, through a combination of X-ray crystallography and electron microscopy. PilMNO forms a dimeric T-shaped structure, binding two copies of the pilin protein at its extremities. The results provide a structural model for the way in which pilin is harvested from the inner membrane and made available to other components of the type IV pilus biogenesis machinery.

Journal Keywords: Chromatography; Affinity; Cloning; Molecular; Crystallography; X-Ray; DNA; Fimbriae; Bacterial; Genetic; Membrane; Microscopy; Electron; Models; Molecular; MultiProtein; Sequence; DNA; Thermus thermophilus

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography