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Reentrant liquid-liquid phase separation in protein solutions at elevated hydrostatic pressures

DOI: 10.1103/PhysRevLett.112.028101 DOI Help
PMID: 24484044 PMID Help

Authors: Johannes Moeller (TU Dortmund) , Sebastian Grobelny (TU Dortmund) , Julian Schulze (TU Dortmund) , Steffen Bieder (TU Dortmund) , Andre Steffen (TU Dortmund) , Mirko Erlkamp (TU Dortmund) , Michael Paulus (TU Dortmund University) , Metin Tolan (TU Dortmund) , Roland Winter (TU Dortmund)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Physical Review Letters , VOL 112 (2) , PAGES 028101

State: Published (Approved)
Published: January 2014
Diamond Proposal Number(s): 7976 , 8604

Abstract: We present results from small-angle x-ray scattering data on the effect of high pressure on the phase behavior of dense lysozyme solutions in the liquid-liquid phase separation region, and characterize the underlying intermolecular protein-protein interactions as a function of temperature and pressure in this region of phase space. A reentrant liquid-liquid phase separation region has been discovered at elevated pressures, which originates in the pressure dependence of the solvent-mediated protein-protein interactions.

Journal Keywords: Liquid-Liquid Phase Separation; Proteins; High Pressure; Small Angle X-Ray Scattering

Subject Areas: Biology and Bio-materials, Chemistry, Physics

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: ID02 at ESRF

Added On: 15/01/2014 08:51

Discipline Tags:

Physics Physical Chemistry Biochemistry Soft condensed matter physics Chemistry Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)