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Coherent X-ray imaging of collagen fibril distributions within intact tendons

DOI: 10.1016/j.bpj.2013.12.016 DOI Help

Authors: Felisa Berenguer (University College London) , Richard J. Bean (University College London) , Laurent Bozec (University College London) , Joan Vila-Comamala (Paul Scherrer Institut) , Fucai Zhang (University of Sheffield) , Cameron M. Kewish (Paul Scherrer Institut) , Oliver Bunk (Paul Scherrer Institut) , John M. Rodenburg (University of Sheffield; Research Complex at Harwell) , Ian K. Robinson (University College London; Research Complex at Harwell)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biophysical Journal , VOL 106 (2) , PAGES 459 - 466

State: Published (Approved)
Published: January 2014

Open Access Open Access

Abstract: The characterization of the structure of highly hierarchical biosamples such as collagen-based tissues at the scale of tens of nanometers is essential to correlate the tissue structure with its growth processes. Coherent x-ray Bragg ptychography is an innovative imaging technique that gives high resolution images of the ordered parts of such samples. Herein, we report how we used this method to image the collagen fibrillar ultrastructure of intact rat tail tendons. The images show ordered fibrils extending over 10–20 ìm in length, with a quantifiable D-banding spacing variation of 0.2%. Occasional defects in the fibrils distribution have also been observed, likely indicating fibrillar fusion events.

Subject Areas: Biology and Bio-materials, Technique Development

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: 34-ID-C at Advanced Photon Source; cSAXS at Swiss Light Source

Added On: 27/01/2014 14:05


Discipline Tags:

Technique Development - Life Sciences & Biotech Biophysics Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)