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Coherent X-Ray Imaging of Collagen Fibril Distributions within Intact Tendons

DOI: 10.1016/j.bpj.2013.12.016 DOI Help

Authors: Felisa Berenguer (London Centre of Nanotechnology) , Richard Bean (London Centre of Nanotechnology) , Laurent Bozec (University College London-Eastman Dental Institute) , Joan Vila-comamala (Diamond Light Source) , Fucai Zhang (Department of Electronic and Electric Engineering, University of Sheffield) , Cameron Kewish (Soleil synchrotron) , Oliver Bunk (Paul Scherrer Institut, Villigen) , John Rodenburg (University of Sheffield) , Ian Robinson (Research Complex at Harwell)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biophysical Journal , VOL 106 (2) , PAGES 459 - 466

State: Published (Approved)
Published: January 2014

Open Access Open Access

Abstract: The characterization of the structure of highly hierarchical biosamples such as collagen-based tissues at the scale of tens of nanometers is essential to correlate the tissue structure with its growth processes. Coherent x-ray Bragg ptychography is an innovative imaging technique that gives high resolution images of the ordered parts of such samples. Herein, we report how we used this method to image the collagen fibrillar ultrastructure of intact rat tail tendons. The images show ordered fibrils extending over 10–20 ìm in length, with a quantifiable D-banding spacing variation of 0.2%. Occasional defects in the fibrils distribution have also been observed, likely indicating fibrillar fusion events.

Subject Areas: Biology and Bio-materials, Technique Development


Instruments: I22-Small angle scattering & Diffraction