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A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes

DOI: 10.1038/nature12907 DOI Help
PMID: 24463512 PMID Help

Authors: Johan Larsbrink (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre) , Theresa E. Rogers (Department of Microbiology and Immunology, University of Michigan Medical School) , Glyn Hemsworth (University of York) , Lauren S. Mckee (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre) , Alexandra S. Tauzin (Michael Smith Laboratories and Department of Chemistry, University of British Columbia) , Oliver Spadiut (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre) , Stefan Klinter (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre) , Nicholas A. Pudlo (Department of Microbiology and Immunology, University of Michigan Medical School) , Karthik Urs (Department of Microbiology and Immunology, University of Michigan Medical School) , Nicole M. Koropatkin (Department of Microbiology and Immunology, University of Michigan Medical School) , A. Louise Creagh (Michael Smith Laboratories and Department of Chemical and Biological Engineering, University of British Columbia) , Charles A. Haynes (Michael Smith Laboratories and Department of Chemical and Biological Engineering, University of British Columbia) , Amelia G. Kelly (Department of Microbiology and Immunology, University of Michigan Medical School) , Stefan Nilsson Cederholm (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre) , Gideon J. Davies (University of York) , Eric C. Martens (Department of Microbiology and Immunology, University of Michigan Medical School) , Harry Brumer (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature

State: Published (Approved)
Published: January 2014

Open Access Open Access

Abstract: The xyloglucans (XyGs) are a ubiquitous family of highly branched plant cell wall polysaccharides5, 6 whose mechanism(s) of degradation in the human gut and consequent importance in nutrition have been unclear1, 7, 8. Here we demonstrate that a single, complex gene locus in Bacteroides ovatus confers XyG catabolism in this common colonic symbiont. Through targeted gene disruption, biochemical analysis of all predicted glycoside hydrolases and carbohydrate-binding proteins, and three-dimensional structural determination of the vanguard endo-xyloglucanase, we reveal the molecular mechanisms through which XyGs are hydrolysed to component monosaccharides for further metabolism

Journal Keywords: Bacteroides; Carbohydrate; Carbohydrate; Cell; Crystallography; X-Ray; Diet; Dietary; Evolution; Molecular; Gastrointestinal; Genetic; Glucans; Glycoside; Humans; Metagenome; Models; Molecular; Phylogeny; Protein; Tertiary; Symbiosis; Xylans

Subject Areas: Biology and Bio-materials, Food Science


Instruments: I03-Macromolecular Crystallography

Added On: 31/01/2014 11:17

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