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Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions

DOI: 10.1016/j.nima.2010.10.135 DOI Help

Authors: Bonnie Wallace (Birkbeck, University of London) , Kunihiko Gekko (Hiroshima Synchrotron Radiation Center, Hiroshima University, Japan) , Søren Vrønning Hoffmann (Institute for Storage Ring Facilities, Aarhus University, Denmark) , Yi-hung Lin (National Synchrotron Radiation Research Centre, Hsinchu) , John C. Sutherland (Brookhaven National Laboratory) , Ye Tao (Institute of High Energy Physics, Chinese Academy of Sciences) , Frank Wien (Synchrotron Soleil) , Robert W Janes (Queen Mary, University of London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nuclear Instruments And Methods In Physics Research Section A: Accelerators, Spectrometers, Detectors And Associated Equipment , VOL 649 (1) , PAGES 177 - 178

State: Published (Approved)
Published: September 2011
Diamond Proposal Number(s): 6084

Abstract: Circular dichroism (CD) spectroscopy is a well-established technique in structural biology. The use of synchrotron radiation as an intense light source for these measurements extends the applications possible using lab-based instruments. In recent years, there has been a major growth in synchrotron radiation circular dichroism (SRCD) beamlines worldwide, including ones at the NSLS, ISA, SRS, HiSOR, BSRF, NSRRC, SOLEIL, Diamond, TERAS, BESSYII, and ANKA synchrotrons. Through the coordinated efforts of beamline scientists and users at these sites, important proof-of-principle studies have been done enabling the method to be developed for novel and productive studies on biological systems. This paper describes the characteristics of SRCD beamlines and some of the new types of applications that have been undertaken using these beamlines.

Journal Keywords: Synchrotron radiation circular dichroism spectroscopy; Protein secondary structure; Conformational changes; Macromolecular interactions

Subject Areas: Technique Development, Biology and Bio-materials, Materials

Instruments: B23-Circular Dichroism

Other Facilities: Hisor BL15 ISA CD1 NSLS U9b NSRRC BL04B SRS CD12 BSRF 4B8 Soleil DISCO

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