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Ferrous iron formation following the co-aggregation of ferric iron and the Alzheimer's disease peptide β-amyloid (1-42)

DOI: 10.1098/rsif.2014.0165 DOI Help
PMID: 24671940 PMID Help

Authors: J. Everett (Keele University) , E. Cespedes (Keele University) , L. R. Shelford (University of Exeter) , C. Exley (Keele University) , J. F. Collingwood (Warwick University) , J. Dobson (University of Florida) , G. Van Der Laan (Diamond Light Source) , C. A. Jenkins (Advanced Light Source, Lawrence Berkeley National Laboratory) , E. Arenholz (Lawrence Berkeley National Laboratory) , N. D. Telling (Keele University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of The Royal Society Interface , VOL 11 (95) , PAGES 20140165 - 20140165

State: Published (Approved)
Published: March 2014

Open Access Open Access

Abstract: For decades, a link between increased levels of iron and areas of Alzheimer's disease (AD) pathology has been recognized, including AD lesions comprised of the peptide β-amyloid (Aβ). Despite many observations of this association, the relationship between Aβ and iron is poorly understood. Using X-ray microspectroscopy, X-ray absorption spectroscopy, electron microscopy and spectrophotometric iron(II) quantification techniques, we examine the interaction between Aβ(1–42) and synthetic iron(III), reminiscent of ferric iron stores in the brain. We report Aβ to be capable of accumulating iron(III) within amyloid aggregates, with this process resulting in Aβ-mediated reduction of iron(III) to a redox-active iron(II) phase. Additionally, we show that the presence of aluminium increases the reductive capacity of Aβ, enabling the redox cycling of the iron. These results demonstrate the ability of Aβ to accumulate iron, offering an explanation for previously observed local increases in iron concentration associated with AD lesions. Furthermore, the ability of iron to form redox-active iron phases from ferric precursors provides an origin both for the redox-active iron previously witnessed in AD tissue, and the increased levels of oxidative stress characteristic of AD. These interactions between Aβ and iron deliver valuable insights into the process of AD progression, which may ultimately provide targets for disease therapies

Journal Keywords: Alzheimer's Disease; ?-Amyloid; W?stite; Redox; X-Ray Absorption

Subject Areas: Biology and Bio-materials, Medicine


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