Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium

DOI: 10.1016/j.bpc.2014.03.002
PMID: 24747675

Authors: Luca Quaroni (Paul Scherrer Institute) , Theodora Zlateva (Paul Scherrer Institute) , Blagoj Sarafimov (Paul Scherrer Institute) , Helen W. Kreuzer (Pacific Northwest National Laboratory) , Katia Wehbe (Diamond Light Source) , Eric L. Hegg (Michigan State University) , Gianfelice Cinque (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biophysical Chemistry , VOL 189 , PAGES 40 - 48

State: Published (Approved)
Published: May 2014
Diamond Proposal Number(s): 5773

Abstract: We successfully tested the viability of using synchrotron-based full-field infrared imaging to study biochemical processes inside living cells. As a model system, we studied fibroblast cells exposed to a medium highly enriched with D2O. We could show that the experimental technique allows us to reproduce at the cellular level measurements that are normally performed on purified biological molecules. We can obtain information about lipid conformation and distribution, kinetics of hydrogen/deuterium exchange, and the formation of concentration gradients of H and O isotopes in water that are associated with cell metabolism. The implementation of the full field technique in a sequential imaging format gives a description of cellular biochemistry and biophysics that contains both spatial and temporal information.

Journal Keywords: Infrared spectromicroscopy; Synchrotron radiation; Single cell; Cell lipid; Isotope tracer; Full-field IR microscopy

Subject Areas: Biology and Bio-materials, Technique Development


Instruments: B22-Multimode InfraRed imaging And Microspectroscopy

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