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Exploiting fast detectors to enter a new dimension in room-temperature crystallography

DOI: 10.1107/S1399004714005379 DOI Help
PMID: 24816094 PMID Help

Authors: Robin Owen (Diamond Light Source) , Neil Paterson (Diamond Light Source) , Danny Axford (Diamond Light Source) , Jun Aishima (Diamond Light Source) , Clemens Schulze-briese (Dectris Ltd) , Jingshan Ren (University of Oxford) , Liz Fry (University of Oxford) , Dave Stuart (Diamond Light Source) , Gwyndaf Evans (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Biological Crystallography , VOL 70 , PAGES 1248 - 1256

State: Published (Approved)
Published: May 2014
Diamond Proposal Number(s): 5968

Open Access Open Access

Abstract: A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerable intensity decay is reached. A simple model accounting for the form of the intensity decay is reintroduced and is applied for the first time to high frame-rate room-temperature data collection.

Journal Keywords: Radiation Damage; Room Temperature; Macromolecular Crystallography; Dose Rate; Crystallography; Crystals; Decay; Dose Rates; Proteins; Radiation Effects

Subject Areas: Technique Development, Biology and Bio-materials

Instruments: I24-Microfocus Macromolecular Crystallography

Added On: 10/06/2014 11:26


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