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The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase fromNectria haematococca

DOI: 10.1111/febs.12778 DOI Help
PMID: 24618038 PMID Help

Authors: Chris Sayer (University of Exeter) , Ruben J. Martinez-torres (Department of Biochemical Engineering, Advanced Centre for Biochemical Engineering, Torrington Place, University College London) , Nina Richter (Department of Chemistry, University College London) , Misha Isupov (University of Exeter) , Helen C. Hailes (Department of Chemistry, University College London) , Jennifer Littlechild (University of Exeter) , John M. Ward (Department of Biochemical Engineering, Advanced Centre for Biochemical Engineering, Torrington Place, University College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Febs Journal , VOL 281 (9) , PAGES 2240 - 2253

State: Published (Approved)
Published: May 2014
Diamond Proposal Number(s): 8889

Open Access Open Access

Abstract: During the last decade the use of transaminases for the production of pharmaceutical and fine chemical intermediates has attracted a great deal of attention. Transaminases are versatile biocatalysts for the efficient production of amine intermediates and many have (S)-enantiospecificity. Transaminases with (R)-specificity are needed to expand the applications of these enzymes in biocatalysis. In this work we have identified a fungal putative (R)-specific transaminase from the Eurotiomycetes Nectria haematococca, cloned a synthetic version of this gene, demonstrated (R)-selective deamination of several substrates including (R)-α-methylbenzylamine, as well as production of (R)-amines, and determined its crystal structure. The crystal structures of the holoenzyme and the complex with an inhibitor gabaculine offer the first detailed insight into the structural basis for substrate specificity and enantioselectivity of the industrially important class of (R)-selective amine : pyruvate transaminases.

Journal Keywords: Biocatalysis ; Cloning ; Molecular ; Genes ; Fungal ; Models ; Molecular ; Nectria ; Protein; Stereoisomerism ; Substrate; Transaminases

Subject Areas: Biology and Bio-materials


Instruments: I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography

Added On: 30/07/2014 13:45

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