Publication

Article Metrics

Citations


Online attention

Analysis of the Hydration Water around Bovine Serum Albumin Using Terahertz Coherent Synchrotron Radiation

DOI: 10.1021/jp407410g DOI Help
PMID: 24328100 PMID Help

Authors: Jordan W. Bye (Department of Chemical & Biological Engineering, ChELSI Institute, University of Sheffield, U.K.) , Stefano Meliga (Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Australia) , Denis Ferachou (Department of Chemical Engineering & Biotechnology, University of Cambridge, U.K.) , Gianfelice Cinque (Diamond Light Source) , J. Axel Zeitler (Department of Chemical Engineering & Biotechnology, University of Cambridge, U.K.) , Robert J. Falconer (Department of Chemical & Biological Engineering, ChELSI Institute, University of Sheffield, U.K.)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Journal Of Physical Chemistry A , VOL 118 (1) , PAGES 83 - 88

State: Published (Approved)
Published: January 2014

Abstract: Terahertz spectroscopy was used to study the absorption of bovine serum albumin (BSA) in water. The Diamond Light Source operating in a low alpha mode generated coherent synchrotron radiation that covered a useable spectral bandwidth of 0.3–3.3 THz (10–110 cm–1). As the BSA concentration was raised, there was a nonlinear change in absorption inconsistent with Beer’s law. At low BSA concentrations (0–1 mM), the absorption remained constant or rose slightly. Above a concentration of 1 mM BSA, a steady decrease in absorption was observed, which was followed by a plateau that started at 2.5 mM. Using a overlapping hydration layer model, the hydration layer was estimated to extend 15 Å from the protein. Calculation of the corrected absorption coefficient (αcorr) for the water around BSA by subtracting the excluded volume of the protein provides an alternative approach to studying the hydration layer that provides evidence for complexity in the population of water around BSA.

Subject Areas: Biology and Bio-materials


Instruments: B22-Multimode InfraRed imaging And Microspectroscopy

Discipline Tags:



Technical Tags: