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A complex gene locus enables xyloglucan utilization in the model saprophyte

DOI: 10.1111/mmi.12776 DOI Help
PMID: 25171165 PMID Help

Authors: Johan Larsbrink (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm, Sweden) , Andrew J. Thompson (Department of Chemistry, University of York, York, UK) , Magnus Lundqvist (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm, Sweden) , Jeffrey G. Gardner (Department of Biological Sciences, University of Maryland – Baltimore County, Baltimore, MD, USA) , Gideon J. Davies (Department of Chemistry, University of York, York, UK) , Harry Brumer (Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm, Sweden)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Molecular Microbiology , VOL 94 (2) , PAGES 418–433

State: Published (Approved)
Published: October 2014
Diamond Proposal Number(s): 7864

Open Access Open Access

Abstract: The degradation of plant biomass by saprophytes is an ecologically important part of the global carbon cycle, which has also inspired a vast diversity of industrial enzyme applications. The xyloglucans (XyGs) constitute a family of ubiquitous and abundant plant cell wall polysaccharides, yet the enzymology of XyG saccharification is poorly studied. Here, we present the identification and molecular characterization of a complex genetic locus that is required for xyloglucan utilization by the model saprophyte Cellvibrio japonicus. In harness, transcriptomics, reverse genetics, enzyme kinetics, and structural biology indicate that the encoded cohort of an á-xylosidase, a â-galactosidase, and an á-l-fucosidase is specifically adapted for efficient, concerted saccharification of dicot (fucogalacto)xyloglucan oligosaccharides following import into the periplasm via an associated TonB-dependent receptor. The data support a biological model of xyloglucan degradation by C. japonicus with striking similarities – and notable differences – to the complex polysaccharide utilization loci of the Bacteroidetes.

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I03-Macromolecular Crystallography

Added On: 24/09/2014 13:51

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