Publication

Article Metrics

Citations


Online attention

Time-resolved crystallography using the Hadamard transform

DOI: 10.1038/nmeth.3139 DOI Help
PMID: 25282611 PMID Help

Authors: Briony Yorke (University of Leeds) , Robin Owen (Diamond Light Source) , Godfrey Beddard (University of Leeds) , Arwen Pearson (University of Leeds)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Methods

State: Published (Approved)
Published: October 2014
Diamond Proposal Number(s): 8367 , 5810

Open Access Open Access

Abstract: We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I24-Microfocus Macromolecular Crystallography