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Impact of residues remote from the catalytic centre on enzyme catalysis of copper nitrite reductase

DOI: 10.1038/ncomms5395 DOI Help
PMID: 25022223 PMID Help

Authors: Nicole G. H. Leferink (Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, U.K.) , Svetlana V. Antonyuk (Molecular Biophysics Group, Faculty of Health and Life Sciences, Institute of Integrative Biology, University of Liverpool, U.K.) , Joseline A. Houwman (Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, U.K) , Nigel S. Scrutton (Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, U.K) , Robert R. Eady (Molecular Biophysics Group, Faculty of Health and Life Sciences, Institute of Integrative Biology, University of Liverpool, U.K.) , Samar Hasnain (Molecular Biophysics Group, Faculty of Health and Life Sciences, Institute of Integrative Biology, University of Liverpool, U.K.)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 5

State: Published (Approved)
Published: March 2014
Diamond Proposal Number(s): 8663

Abstract: Enzyme mechanisms are often probed by structure-informed point mutations and measurement of their effects on enzymatic properties to test mechanistic hypotheses. In many cases, the challenge is to report on complex, often inter-linked elements of catalysis. Evidence for long-range effects on enzyme mechanism resulting from mutations remains sparse, limiting the design/redesign of synthetic catalysts in a predictable way. Here we show that improving the accessibility of the active site pocket of copper nitrite reductase by mutation of a surface-exposed phenylalanine residue (Phe306), located 12Å away from the catalytic site type-2 Cu (T2Cu), profoundly affects intra-molecular electron transfer, substrate-binding and catalytic activity. Structures and kinetic studies provide an explanation for the lower affinity for the substrate and the alteration of the rate-limiting step in the reaction. Our results demonstrate that distant residues remote from the active site can have marked effects on enzyme catalysis, by driving mechanistic change through relatively minor structural perturbations.

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I24-Microfocus Macromolecular Crystallography

Other Facilities: SOLEIL