Publication

Article Metrics

Citations


Online attention

Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1

DOI: 10.1107/S139900471401339X DOI Help
PMID: 25084338 PMID Help

Authors: Kamel El Omari (Division of Structural Biology, University of Oxford, U.K.) , Oleg Iourin (Division of Structural Biology, University of Oxford, U.K.) , Jan Kadlec (Division of Structural Biology, University of Oxford, U.K.) , Richard Fearn (Diamond Light Source) , Dave Hall (Diamond Light Source) , Karl Harlos (Division of Structural Biology, University of Oxford, U.K.) , Jonathan M. Grimes (Division of Structural Biology, University of Oxford, U.K., Diamond Light Source) , David I. Stuart (Division of Structural Biology, University of Oxford, U.K., Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Biological Crystallography , VOL 70 , PAGES 2197 - 2203

State: Published (Approved)
Published: August 2014
Diamond Proposal Number(s): 8423

Open Access Open Access

Abstract: Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffracted very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 Å resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 Å resolution model building was achievable.

Journal Keywords: Atoms; Crystal Structure; Crystals; Density; Derivatization; Mathematical Solutions; Resolution; Signals; Solutions; Sulfur; Wavelengths

Subject Areas: Biology and Bio-materials, Technique Development


Instruments: I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography