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Detection of interaction between protein trytophan residues and small or macromolecular ligands by synchrotron radiation magnetic circular dichroism

DOI: 10.1039/C4AY02618G DOI Help

Authors: Ashley Hughes (University of Liverpool) , Timothy R. Rudd (Diamond Light Source) , Gareth S. A. Wright (University of Liverpool) , David Garth Fernig (University of Liverpool) , Giuliano Siligardi (Diamond Light Source) , Edwin Yates (University of Liverpool, Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Analytical Methods

State: Published (Approved)
Published: December 2014
Diamond Proposal Number(s): 9218 , 5843

Abstract: Presented herein is a novel method for the detection of tryptophan-ligand interactions utilising magnetic circular dichroism. Two examples are presented, one a small molecule and another, a large polysaccharide, heparan sulfate. These data are also the first reported example of a Trp:glycosaminoglycan interaction shown by magnetic circular dichroism.

Subject Areas: Chemistry, Technique Development, Biology and Bio-materials

Instruments: B23-Circular Dichroism

Added On: 16/12/2014 11:57

Discipline Tags:

Biochemistry Technique Development - Chemistry Technique Development - Life Sciences & Biotech Chemistry Life Sciences & Biotech

Technical Tags:

Spectroscopy Circular Dichroism (CD)