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Application of In Situ Diffraction in High-Throughput Structure Determination Platforms

DOI: 10.1007/978-1-4939-2230-7_13 DOI Help
PMID: 25502203 PMID Help

Authors: Raymond J. Owens (Diamond Light Source) , Pierre Aller (Diamond Light Source) , Juan Sanchez-weatherby (Diamond Light Source) , James Foadi (Diamond Light Source) , Graeme Winter (Diamond Light Source) , Carina Lobley (Diamond Light Source) , Danny Axford (Diamond Light Source) , Alun Ashton (Diamond Light Source) , Domenico Bellini (Diamond Light Source) , Jose Brandao-neto (Diamond Light Source) , Simone Culurgioni (Diamond Light Source) , Alice Douangamath (Diamond Light Source) , Ramona Duman (Diamond Light Source) , Gwyndaf Evans (Diamond Light Source) , Stuart Fisher (Diamond Light Source) , Ralf Flaig (Diamond Light Source) , Dave Hall (Diamond) , Petra Lukacik (Diamond Light Source) , Marco Mazzorana (Diamond Light Source) , Katherine Mcauley (Diamond Light Source)
Co-authored by industrial partner: No

Type: Book Chapter

State: Published (Approved)
Published: November 2015

Abstract: Macromolecular crystallography (MX) is the most powerful technique available to structural biologists to visualize in atomic detail the macromolecular machinery of the cell. Since the emergence of structural genomics initiatives, significant advances have been made in all key steps of the structure determination process. In particular, third-generation synchrotron sources and the application of highly automated approaches to data acquisition and analysis at these facilities have been the major factors in the rate of increase of macromolecular structures determined annually. A plethora of tools are now available to users of synchrotron beamlines to enable rapid and efficient evaluation of samples, collection of the best data, and in favorable cases structure solution in near real time. Here, we provide a short overview of the emerging use of collecting X-ray diffraction data directly from the crystallization experiment. These in situ experiments are now routinely available to users at a number of synchrotron MX beamlines. A practical guide to the use of the method on the MX suite of beamlines at Diamond Light Source is given.

Subject Areas: Biology and Bio-materials, Technique Development

Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Added On: 13/01/2015 14:59

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