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Temperature and pressure tuneable swollen bicontinuous cubic phases approaching nature's length scales

DOI: 10.1039/C4SM02343A DOI Help

Authors: Hanna Barriga (Imperial College London) , Arwen Tyler (Imperial College London) , Nicola Mccarthy (Imperial College London) , E. S. Parsons (Imperial College London) , Oscar Ces (Imperial College London) , Robert Law (Imperial College London) , John Seddon (Imperial College London) , Nick Brooks (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Soft Matter , VOL 11 (3) , PAGES 600 - 607

State: Published (Approved)
Published: November 2014
Diamond Proposal Number(s): 9756

Open Access Open Access

Abstract: Bicontinuous cubic structures offer enormous potential in applications ranging from protein crystallisation to drug delivery systems and have been observed in cellular membrane structures. One of the current bottlenecks in understanding and exploiting these structures is that cubic scaffolds produced in vitro are considerably smaller in size than those observed in biological systems, differing by almost an order of magnitude in some cases. We have addressed this technological bottleneck and developed a methodology capable of manufacturing highly swollen bicontinuous cubic membranes with length scales approaching those seen in vivo. Crucially, these cubic systems do not require the presence of proteins. We have generated highly swollen Im3m symmetry bicontinuous cubic phases with lattice parameters of up to 480 Angstrom, composed of ternary mixtures of monoolein, cholesterol and negatively charged lipid (DOPS or DOPG) and we have been able to tune their lattice parameters. The swollen cubic phases are highly sensitive to both temperature and pressure; these structural changes are likely to be controlled by a fine balance between lipid headgroup repulsions and lateral pressure in the hydrocarbon chain region.

Subject Areas: Chemistry, Biology and Bio-materials

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: ESRF

Added On: 29/01/2015 14:39

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