Article Metrics


Online attention

Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from

DOI: 10.1107/S2053230X14025278 DOI Help
PMID: 25484229 PMID Help

Authors: Sofiyah Shaibullah (Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM)) , Nurhikmah Mohd-sharif (Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM)) , Kok Lian Ho (Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM)) , Mohd Firdaus-raih (Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM)) , Sheila Nathan (School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM)) , Rahmah Mohamed (INTI International University, Bandar Baru Nilai) , Chyan Leong Ng (Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM))
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 70 , PAGES 1697 - 1700

State: Published (Approved)
Published: December 2014

Abstract: Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ~25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 Å resolution. It belonged to space group C2221, with unit-cell parameters a = 85.36, b = 115.63, c = 46.73 Å. The calculated Matthews coefficient (VM) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%.

Journal Keywords: Burkholderia pseudomallei; BPSL1038; hypothetical protein

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I02-Macromolecular Crystallography

Discipline Tags:

Technical Tags: