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The YmdB Phosphodiesterase Is a Global Regulator of Late Adaptive Responses in Bacillus subtilis

DOI: 10.1128/JB.00826-13 DOI Help
PMID: 24163345 PMID Help

Authors: Christine Diethmaier (Department of General Microbiology, Georg August University Göttingen) , Joseph A Newman (Institute for Cell and Molecular Biosciences, Newcastle University) , Akos T. Kovacs (Department of Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute) , Volkhard Kaever (Research Core Unit for Mass Spectrometry-Metabolomics and Institute of Pharmacology, Hannover Medical School) , Christina Herzberg (Department of General Microbiology, Georg August University Göttingen) , Cecilia Rodrigues (Institute for Cell and Molecular Biosciences, Newcastle University) , Mirjam Boonstra (Department of Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute) , Oscar P. Kuipers (Department of Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute) , Rick Lewis (Institute for Cell and Molecular Biosciences, Newcastle University) , Jorg Stulke (Department of General Microbiology, Georg August University Göttingen)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Bacteriology , VOL 196 (2) , PAGES 265 - 275

State: Published (Approved)
Published: December 2013

Abstract: Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and are deficient in SlrR, a SinR antagonist. Here, we report the functional and structural characterization of YmdB, and we find that YmdB is a phosphodiesterase with activity against 2' 3' and 3' 5 'cyclic nucleotide monophosphates. The structure of YmdB reveals that the enzyme adopts a conserved phosphodiesterase fold with a binuclear metal center. Mutagenesis of a catalytically crucial residue demonstrates that the enzymatic activity of YmdB is essential for biofilm formation. The deletion of ymdB affects the expression of more than 800 genes; the levels of the sigma D-dependent motility regulon and several sporulation genes are increased, and the levels of the SinR-repressed biofilm genes are decreased, confirming the role of YmdB in regulating late adaptive responses of B. subtilis.

Journal Keywords: Biofilms ; Crystallography ; X-Ray ; DNA; Gene; Gene; Bacterial ; Models ; Molecular ; Phosphoric; Protein Conformation

Subject Areas: Biology and Bio-materials


Instruments: I02-Macromolecular Crystallography

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