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A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis

DOI: 10.1042/BSR20140143 DOI Help
PMID: 25372605 PMID Help

Authors: Eva Ullmann (Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering) , Tien-chye Tan (Department of Medical Biochemistry and Biophysics, Karolinska Institutet) , Thomas Gundinger (Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering) , Christoph Herwig (Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering) , Christina Divne (Department of Medical Biochemistry and Biophysics, Karolinska Institutet) , Oliver Spadiut (Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Bioscience Reports , VOL 34 , PAGES 893 - 904

State: Published (Approved)
Published: November 2014
Diamond Proposal Number(s): 8492

Open Access Open Access

Abstract: Methanothermobacter marburgensis is a strictly anaerobic, thermophilic methanogenic archaeon that uses methanogenesis to convert H2 and CO2 to energy. M. marburgensis is one of the best-studied methanogens, and all genes required for methanogenic metabolism have been identified. Nonetheless, the present study describes a gene (Gene ID 9704440) coding for a putative NAD(P)H:quinone oxidoreductase that has not yet been identified as part of the metabolic machinery. The gene product, MmNQO, was successfully expressed, purified and characterized biochemically, as well as structurally. MmNQO was identified as a flavin-dependent NADH:quinone oxidoreductase with the capacity to oxidize NADH in the presence of a wide range of electron acceptors, whereas NADPH was oxidized with only three acceptors. The 1.50 Å crystal structure of MmNQO features a homodimeric enzyme where each monomer comprises 196 residues folding into flavodoxin-like α/β domains with non-covalently bound FMN (flavin mononucleotide). The closest structural homologue is the modulator of drug activity B from Streptococcus mutans with 1.6 Å root-mean-square deviation on 161 Cα atoms and 28% amino-acid sequence identity. The low similarity at sequence and structural level suggests that MmNQO is unique among NADH:quinone oxidoreductases characterized to date. Based on preliminary bioreactor experiments, MmNQO could provide a useful tool to prevent overflow metabolism in applications that require cells with high energy demand.

Subject Areas: Biology and Bio-materials


Instruments: I03-Macromolecular Crystallography

Added On: 24/02/2015 09:50

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