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Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-beta-1,4-glucanase B (CelB) from Bacillus halodurans

DOI: 10.1107/S2053230X14008395 DOI Help
PMID: 24915086 PMID Help

Authors: Immacolata Venditto (CIISA-Faculdade de Medicina Veterinaria, Universidade de Lisboa) , Helena Santos (CIISA-Faculdade de Medicina Veterinaria, Universidade de Lisboa) , Luís M. A. Ferreira (CIISA-Faculdade de Medicina Veterinaria, Universidade de Lisboa) , Kazuo Sakka (Graduate School of Bioresources, Mie University) , Carlos M. G. A. Fontes (CIISA-Faculdade de Medicina Veterinaria, Universidade de Lisboa) , Shabir Najmudin (CIISA-Faculdade de Medicina Veterinaria, Universidade de Lisboa)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 70 , PAGES 754 - 757

State: Published (Approved)
Published: June 2014
Diamond Proposal Number(s): 8425

Abstract: Plant cell-wall polysaccharides offer an abundant energy source utilized by many microorganisms, thus playing a central role in carbon recycling. Aerobic microorganisms secrete carbohydrate-active enzymes (CAZymes) that catabolize this composite structure, comprising cellulose, hemicellulose and lignin, into simple compounds such as glucose. Carbohydrate-binding modules (CBMs) enhance the efficacy of associated CAZYmes. They are organized into families based on primary-sequence homology. CBM family 46 contains more than 40 different members, but has yet to be fully characterized. Here, a recombinant derivative of the C-terminal family 46 CBM module (BhCBM46) of Bacillus halodurans endo-[beta]-1,4-glucanase B (CelB) was overexpressed in Escherichia coli and purified by immobilized metal-ion affinity chromatography. Preliminary structural characterization was carried out on BhCBM46 crystallized in different conditions. The crystals of BhCBM46 belonged to the tetragonal space group I4122. Data were collected for the native form and a selenomethionine derivative to 2.46 and 2.3 Å resolution, respectively. The BhCBM46 structure was determined by a single-wavelength anomalous dispersion experiment using AutoSol from the PHENIX suite.

Subject Areas: Biology and Bio-materials

Instruments: I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography

Added On: 25/02/2015 07:44

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