Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34

DOI: 10.1107/S2053230X14010449
PMID: 25005101

Authors: Meritxell Granell (Centro Nacional de Biotecnologia (CNB-CSIC)) , Mikiyoshi Namura (Tokyo Institute of Technology) , Sara Alvira (Centro Nacional de Biotecnologia (CNB-CSIC)) , Carmela Garcia-Doval (Centro Nacional de Biotecnologia (CNB-CSIC)) , Abhimanyu Singh (Centro Nacional de Biotecnologia (CNB-CSIC)) , Irina Gutsche (Tokyo Institute of Technology) , Mark J. Van Raaij (Centro Nacional de Biotecnologia (CNB-CSIC)) , Shuji Kanamaru (Tokyo Institute of Technology)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 70 , PAGES 970 - 975

State: Published (Approved)
Published: July 2014
Diamond Proposal Number(s): 5860

Abstract: The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 Å resolution, crystals of gp34(781-1289) diffracting to 1.9 Å resolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 Å resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.

Journal Keywords: bacteriophage T4; gene product 34 (gp34)

Diamond Keywords: Bacteriophages

Subject Areas: Biology and Bio-materials


Instruments: I04-Macromolecular Crystallography

Other Facilities: ESRF, DESY, ALBA

Added On: 25/02/2015 09:53

Discipline Tags:

Genetics Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)