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Pressure-temperature phase behavior of mixtures of natural sphingomyelin and ceramide extracts

DOI: 10.1021/la504935c DOI Help

Authors: Hanna Barriga (Imperial College London) , Edward Parsons (Imperial College London) , Nicola Mccarthy (Imperial College London) , Oscar Ces (Imperial College London) , John Seddon (Imperial College London) , Robert Law (Imperial College London) , Nick Brooks (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Langmuir , VOL 31 (12)

State: Published (Approved)
Published: March 2015
Diamond Proposal Number(s): 8366 , 9150 , 9756

Abstract: Ceramides are a group of sphingolipids that act as highly important signaling molecules in a variety of cellular processes including differentiation and apoptosis. The predominant in vivo synthetic pathway for ceramide formation is via sphingomyelinase catalyzed hydrolysis of sphingomyelin. The biochemistry of this essential pathway has been studied in detail; however, there is currently a lack of information on the structural behavior of sphingomyelin- and ceramide-rich model membrane systems, which is essential for developing a bottom-up understanding of ceramide signaling and platform formation. We have studied the lyotropic phase behavior of sphingomyelin−ceramide mixtures in excess water as a function of temperature (30−70 °C) and pressure (1−200 MPa) by small- and wide-angle X-ray scattering. At low ceramide concentrations the mixtures form the ripple gel phase (Pβ′) below the gel transition temperature for sphingomyelin, and this observation has been confirmed by atomic force microscopy. Formation of the ripple gel phase can also be induced at higher temperatures via the application of hydrostatic pressure. At high ceramide concentration an inverse hexagonal phase (HII) is formed coexisting with a cubic phase.

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: N/A

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