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Discrimination between two different grades of human glioma based on blood vessel infrared spectral imaging

DOI: 10.1007/s00216-015-8891-z DOI Help
PMID: 26168973 PMID Help

Authors: Katia Wehbe (Diamond Light Source) , Isabelle Forfar (University of Bordeaux) , Sandrine Eimer (Department of Pathology, Pellegrin University Hospital) , Gianfelice Cinque (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Analytical And Bioanalytical Chemistry

State: Published (Approved)
Published: July 2015
Diamond Proposal Number(s): 7138 , 8106

Open Access Open Access

Abstract: Gliomas are brain tumours classified into four grades with increasing malignancy from I to IV. The development and the progression of malignant glioma largely depend on the tumour vascularization. Due to their tissue heterogeneity, glioma cases can be difficult to classify into a specific grade using the gold standard of histological observation, hence the need to base classification on a quantitative and reliable analytical method for accurately grading the disease. Previous works focused specifically on vascularization study by Fourier transform infrared (FTIR) spectroscopy, proving this method to be a way forward to detect biochemical changes in the tumour tissue not detectable by visual techniques. In this project, we employed FTIR imaging using a focal plane array (FPA) detector and globar source to analyse large areas of glioma tumour tissue sections via molecular fingerprinting in view of helping to define markers of the tumour grade. Unsupervised multivariate analysis (hierarchical cluster analysis and principal component analysis) of blood vessel spectral data, retrieved from the FPA images, revealed the fine structure of the borderline between two areas identified by a pathologist as grades III and IV. Spectroscopic indicators are found capable of discriminating different areas in the tumour tissue and are proposed as biomolecular markers for potential future use of grading gliomas.

Journal Keywords: Glioma; Grading; Blood Vessels; Ftir Spectroscopy; Fpa Imaging

Subject Areas: Chemistry, Biology and Bio-materials

Diamond Offline Facilities: FTIR
Instruments: B22-Multimode InfraRed imaging And Microspectroscopy