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Improved crystallization and diffraction of caffeine-induced death suppressor protein 1 (Cid1)

DOI: 10.1107/S2053230X15001351 DOI Help
PMID: 25760713 PMID Help

Authors: Luke A. Yates (Wellcome Trust Centre for Human Genetics, University of Oxford) , Benjamin P. Durrant (Wellcome Trust Centre for Human Genetics, University of Oxford) , Michael Barber (Wellcome Trust Centre for Human Genetics, University of Oxford) , Karl Harlos (Wellcome Trust Centre for Human Genetics, University of Oxford) , Sophie Fleurdépine (University of Oxford) , Chris J. Norbury (University of Oxford) , Robert Gilbert (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 71 (3) , PAGES 346 - 353

State: Published (Approved)
Published: March 2015
Diamond Proposal Number(s): 10627

Open Access Open Access

Abstract: The post-transcriptional addition of uridines to the 3'-end of RNAs is an important regulatory process that is critical for coding and noncoding RNA stability. In fission yeast and metazoans this untemplated 3'-uridylylation is catalysed by a single family of terminal uridylyltransferases (TUTs) whose members are adapted to specific RNA targets. In Schizosaccharomyces pombe the TUT Cid1 is responsible for the uridylylation of polyadenylated mRNAs, targeting them for destruction. In metazoans, the Cid1 orthologues ZCCHC6 and ZCCHC11 uridylate histone mRNAs, targeting them for degradation, but also uridylate microRNAs, altering their maturation. Cid1 has been studied as a model TUT that has provided insights into the larger and more complex metazoan enzyme system. In this paper, two strategies are described that led to improvements both in the crystallogenesis of Cid1 and in the resolution of diffraction by ~1.5 Å. These advances have allowed high-resolution crystallo­graphic studies of this TUT system to be initiated.

Journal Keywords: Terminal Uridylyltransferases; Cid1

Diamond Keywords: Enzymes

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Added On: 23/09/2015 12:16


Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)