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Structural characterization of the principal mRNA-export factor Mex67–Mtr2 from Chaetomium thermophilum

DOI: 10.1107/S2053230X15008766 DOI Help
PMID: 26144233 PMID Help

Authors: Shintaro Aibara (MRC Laboratory of Molecular Biology) , Eugene Valkov (MRC Laboratory of Molecular Biology, Cambridge, U.K.) , Meindert Lamers (MRC Laboratory of Molecular Biology) , Lyudmila Dimitrova (MRC) , Ed Hurt (MRC) , Murray Stewart (MRC)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 71 (7) , PAGES 876 - 888

State: Published (Approved)
Published: July 2015
Diamond Proposal Number(s): 8547

Open Access Open Access

Abstract: Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67–Mtr2 that facilitate mRNA nuclear export.

Journal Keywords: Nuclear Transport; Mex67; Chaetomium Thermophilum; Rna Binding

Subject Areas: Biology and Bio-materials

Instruments: I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Added On: 28/09/2015 13:04

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