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Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein
Authors:
Samuel
Reddington
(Cardiff University)
,
Sarunas
Driezis
(Cardiff University)
,
Andrew
Hartley
(Cardiff University)
,
Peter D.
Watson
(Cardiff University)
,
Pierre J.
Rizkallah
(Cardiff University)
,
Dafydd
Jones
(Cardiff University)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Rsc Advances
, VOL 5 (95)
, PAGES 77734 - 77738
State:
Published (Approved)
Published:
September 2015
Diamond Proposal Number(s):
10462

Abstract: The photochemical properties of phenyl azide have been exploited to modulate the function of a red autofluorescent protein, mCherry. Using genetic code reprogramming, phenyl azide chemistry has been introduced at functionally strategic positions in mCherry leading to deactivation, activation or enhancement upon UV irradiation.
Subject Areas:
Biology and Bio-materials,
Chemistry
Instruments:
I03-Macromolecular Crystallography
,
I04-Macromolecular Crystallography
Added On:
12/10/2015 19:58
Documents:
c5ra13552d.pdf
Discipline Tags:
Biochemistry
Chemistry
Structural biology
Life Sciences & Biotech
Technical Tags:
Diffraction
Macromolecular Crystallography (MX)