Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein

DOI: 10.1039/C5RA13552D

Authors: Samuel Reddington (Cardiff University) , Sarunas Driezis (Cardiff University) , Andrew Hartley (Cardiff University) , Peter D. Watson (Cardiff University) , Pierre J. Rizkallah (Cardiff University) , Dafydd Jones (Cardiff University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Rsc Advances , VOL 5 (95) , PAGES 77734 - 77738

State: Published (Approved)
Published: September 2015
Diamond Proposal Number(s): 10462

Abstract: The photochemical properties of phenyl azide have been exploited to modulate the function of a red autofluorescent protein, mCherry. Using genetic code reprogramming, phenyl azide chemistry has been introduced at functionally strategic positions in mCherry leading to deactivation, activation or enhancement upon UV irradiation.

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Added On: 12/10/2015 19:58

Documents:
c5ra13552d.pdf

Discipline Tags:

Biochemistry Chemistry Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)