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Determination of the structure and morphology of gold nanoparticle–HSA protein complexes

DOI: 10.1039/C5NR05147A DOI Help

Authors: Robin Capomaccio (European Commission, Joint Research Centre, Institute for Health and Consumer Protection; BMSSI-UMR 5086, Université Lyon 1,) , Isaac Ojea-Jimenez (European Commission, Joint Research Centre, Institute for Health and Consumer Protection) , Pascal Colpo (European Commission, Joint Research Centre, Institute for Health and Consumer Protection) , Douglas Gilliland (European Commission, Joint Research Centre, Institute for Health and Consumer Protection) , Giacomo Ceccone (European Commission, Joint Research Centre, Institute for Health and Consumer Protection) , François Rossi (European Commission, Joint Research Centre, Institute for Health and Consumer Protection) , Luigi Calzolai (European Commission, Joint Research Centre, Institute for Health and Consumer Protection)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nanoscale

State: Published (Approved)
Published: October 2015
Diamond Proposal Number(s): 9064 , 9836

Open Access Open Access

Abstract: We propose a simple method to determine the structure and morphology of nanoparticle protein complexes. By combining a separation method with online size measurements, density measurements and circular dichroism, we could identify the number of proteins bound to each nanoparticle and their secondary structure changes in the complex. This method provides much-needed experimental information on the interaction of proteins with nanoparticles and on the behavior of nanoparticles in biological systems

Journal Keywords: Circular Dichroism; Nanoparticle; Size Fractionation; Fff

Subject Areas: Materials, Biology and Bio-materials, Technique Development


Instruments: B23-Circular Dichroism

Added On: 18/10/2015 15:30

Documents:
c5nr05147a.pdf

Discipline Tags:

Technique Development - Life Sciences & Biotech Materials Science Nanoscience/Nanotechnology Life Sciences & Biotech

Technical Tags:

Spectroscopy Circular Dichroism (CD)