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Automation and Experience of Controlled Crystal Dehydration: Results from the European Synchrotron HC1 Collaboration

DOI: 10.1021/cg500890r DOI Help

Authors: Matthew W. Bowler (European Molecular Biology Laboratory) , Uwe Mueller (Macromolecular Crystallography (HZB-MX), Helmholtz-Zentrum Berlin) , Manfred S. Weiss (Macromolecular Crystallography (HZB-MX), Helmholtz-Zentrum Berlin) , Juan Sanchez-weatherby (Diamond Light Source) , Thomas Sorensen (Diamond Light Source) , Marjolein M. G. M. Thunnissen (The MAX IV Laboratory, Lund University) , Thomas Ursby (The MAX IV Laboratory, Lund University) , Alexandre Gobbo (European Molecular Biology Laboratory) , Silvia Russi (European Molecular Biology Laboratory) , Michael G. Bowler (Department of Physics, University of Oxford) , Sandor Brockhauser (European Molecular Biology Laboratory) , Olof Svensson (ESRF) , Florent Cipriani (European Molecular Biology Laboratory)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Crystal Growth & Design , VOL 15 (3) , PAGES 1043 - 1054

State: Published (Approved)
Published: March 2015

Open Access Open Access

Abstract: Controlled dehydration of macromolecular crystals can lead to significant improvements in crystalline order, which often manifests itself in higher diffraction quality. Devices that can accurately control the humidity surrounding crystals on a beamline have led to this technique being increasingly adopted as experiments become easier and more reproducible. However, these experiments are often carried out by trial and error, and in order to facilitate and streamline them four European synchrotrons have established a collaboration around the HC1b dehydration device. The MAX IV Laboratory, Diamond Light Source, BESSY II, and the EMBL Grenoble Outstation/ ESRF have pooled information gathered from user experiments, and on the use of the device, to propose a set of guidelines for these experiments. Here, we present the status and automation of the installations, advice on how best to perform experiments using the device, and an analysis of successful experiments that begins to show some trends in the type of protocols required by some systems. The dehydration methods shown are applicable to any device that allows control of the relative humidity of the air surrounding a macromolecular crystal.

Subject Areas: Chemistry

Technical Areas: Controls