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A four-helix bundle stores copper for methane oxidation

DOI: 10.1038/nature14854 DOI Help
PMID: 26308900 PMID Help

Authors: Nicolas Vita (Newcastle University) , Semeli Platsaki (Newcastle University) , Arnaud Baslé (Newcastle University) , Stephen J. Allen (Newcastle University) , Neil G. Paterson (Diamond Light Source) , Andrew T. Crombie (University of East Anglia) , J. Colin Murrell (University of East Anglia) , Kevin J. Waldron (Newcastle University) , Christopher Dennison (Newcastle University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature , VOL 525 (7567) , PAGES 140 - 143

State: Published (Approved)
Published: August 2015

Abstract: Methane-oxidizing bacteria (methanotrophs) require large quantities of copper for the membrane-bound (particulate) methane monooxygenase1, 2. Certain methanotrophs are also able to switch to using the iron-containing soluble methane monooxygenase to catalyse methane oxidation, with this switchover regulated by copper3, 4. Methane monooxygenases are nature’s primary biological mechanism for suppressing atmospheric levels of methane, a potent greenhouse gas. Furthermore, methanotrophs and methane monooxygenases have enormous potential in bioremediation and for biotransformations producing bulk and fine chemicals, and in bioenergy, particularly considering increased methane availability from renewable sources and hydraulic fracturing of shale rock5, 6. Here we discover and characterize a novel copper storage protein (Csp1) from the methanotroph Methylosinus trichosporium OB3b that is exported from the cytosol, and stores copper for particulate methane monooxygenase. Csp1 is a tetramer of four-helix bundles with each monomer binding up to 13 Cu(I) ions in a previously unseen manner via mainly Cys residues that point into the core of the bundle. Csp1 is the first example of a protein that stores a metal within an established protein-folding motif. This work provides a detailed insight into how methanotrophs accumulate copper for the oxidation of methane. Understanding this process is essential if the wide-ranging biotechnological applications of methanotrophs are to be realized. Cytosolic homologues of Csp1 are present in diverse bacteria, thus challenging the dogma that such organisms do not use copper in this location.

Journal Keywords: Metalloproteins; X-Ray Crystallography

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Added On: 02/11/2015 11:38

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