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The effect of hydrostatic pressure on model membrane domain composition and lateral compressibility

DOI: 10.1039/C5CP04239A DOI Help

Authors: Hanna M. G. Barriga (Imperial College London) , Robert V. Law (Imperial College London) , John Seddon (Imperial College London) , Oscar Ces (Imperial College London) , Nicholas J. Brooks (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Physical Chemistry Chemical Physics

State: Published (Approved)
Published: November 2015
Diamond Proposal Number(s): 9150 , 9756

Abstract: Phase separation in ternary model membranes is known to occur over a range of temperatures and compositions and can be induced by increasing hydrostatic pressure. We have used small angle X-ray scattering (SAXS) to study phase separation along pre-determined tie lines in dioleoylphosphatidylcholine (DOPC), dipalmitoylphosphatidylcholine (DPPC) and cholesterol (CHOL) mixtures. We can unequivocally distinguish the liquid ordered (Lo) and liquid disordered (Ld) phases in diffraction patterns from biphasic mixtures and compare their lateral compressibility. The variation of tie line endpoints with increasing hydrostatic pressure was determined, at atmospheric pressure and up to 100 MPa. We find an extension and shift of the tie lines towards the DOPC rich region of the phase diagram at increased pressure, this behaviour differs slightly from that reported for decreasing temperature.

Journal Keywords: High Pressure; Membranes; Domain Formation

Subject Areas: Chemistry, Biology and Bio-materials

Instruments: I22-Small angle scattering & Diffraction

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