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The Scottish Structural Proteomics Facility: targets, methods and outputs

DOI: 10.1007/s10969-010-9090-y DOI Help
PMID: 20419351 PMID Help

Authors: Muse Oke (University of St. Andrews) , Lester G. Carter (University of St. Andrews) , Kenneth A. Johnson (University of St. Andrews) , Huanting Liu (University of St. Andrews) , Stephen A. Mcmahon (University of St. Andrews) , Xuan Yan (University of St. Andrews) , Melina Kerou (University of St. Andrews) , Nadine D. Weikart (University of St. Andrews) , Nadia Kadi (University of St. Andrews) , Md. Arif Sheikh (University of St. Andrews) , Stefan Schmelz (University of St. Andrews) , Mark Dorward (University of St. Andrews) , Michal Zawadzki (University of St. Andrews) , Christopher Cozens (University of St. Andrews) , Helen Falconer (University of St. Andrews) , Helen Powers (University of St. Andrews) , Ian M. Overton (University of St. Andrews) , C. A. Johannes Van Niekerk (University of St. Andrews) , Xu Peng (University of St. Andrews) , James Naismith (University of St. Andrews)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Structural And Functional Genomics , VOL 11 , PAGES 167 - 180

State: Published (Approved)
Published: September 2010

Open Access Open Access

Abstract: The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.

Journal Keywords: Crystallization; Humans; Laboratories; Proteins; Proteomics; Scotland

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography