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Structure determination of VEGF-E by sulfur SAD
DOI:
10.1107/S0907444906036742
PMID:
17057349
Authors:
Armin
Wagner
(Diamond Light Source)
,
Michel
Pieren
(Biomolecular Research, Paul Scherrer Institut, Villigen-PSI, Switzerland)
,
Clemens
Schulze- Briese
(Swiss Light Source, Paul Scherrer Institut, Villigen-PSI, Switzerland)
,
Kurt
Ballmer-hofer
(Biomolecular Research, Paul Scherrer Institut, Villigen-PSI, Switzerland)
,
Andrea
Prota
(Biomolecular Research, Paul Scherrer Institut, Villigen-PSI, Switzerland)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Acta Crystallographica Section D Biological Crystallography
, VOL D62
, PAGES 1430–-1434
State:
Published (Approved)
Published:
November 2006
Abstract: The crystal structure of VEGF-E was solved by the sulfur single-wavelength anomalous dispersion method (S-SAD) using highly redundant low-resolution data collected at a wavelength of 1.7 Å with an estimated anomalous signal of 1.5%. 11 sulfur sites, nine out of 16 disulfide bonds and two out of 12 methionines could be located in the asymmetric unit using data truncated at a resolution of 4.1 Å; however, none of the common diffraction data-quality indicators for SAD allowed clear discrimination between successful and unsuccessful resolution cutoffs. The high solvent content of 75% allowed efficient density modification to be performed and an unbiased electron-density map of good quality to be generated. This study demonstrates the strength of S-¬SAD for phasing using accurate highly redundant data at low resolution.
Subject Areas:
Biology and Bio-materials
Facility: SLS
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