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Structure determination of VEGF-E by sulfur SAD

DOI: 10.1107/S0907444906036742 DOI Help
PMID: 17057349 PMID Help

Authors: Armin Wagner (Diamond Light Source) , Michel Pieren (Biomolecular Research, Paul Scherrer Institut, Villigen-PSI, Switzerland) , Clemens Schulze- Briese (Swiss Light Source, Paul Scherrer Institut, Villigen-PSI, Switzerland) , Kurt Ballmer-Hofer (Biomolecular Research, Paul Scherrer Institut, Villigen-PSI, Switzerland) , Andrea Prota (Biomolecular Research, Paul Scherrer Institut, Villigen-PSI, Switzerland)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Biological Crystallography , VOL D62 , PAGES 1430–-1434

State: Published (Approved)
Published: November 2006

Abstract: The crystal structure of VEGF-E was solved by the sulfur single-wavelength anomalous dispersion method (S-SAD) using highly redundant low-resolution data collected at a wavelength of 1.7 Å with an estimated anomalous signal of 1.5%. 11 sulfur sites, nine out of 16 disulfide bonds and two out of 12 methionines could be located in the asymmetric unit using data truncated at a resolution of 4.1 Å; however, none of the common diffraction data-quality indicators for SAD allowed clear discrimination between successful and unsuccessful resolution cutoffs. The high solvent content of 75% allowed efficient density modification to be performed and an unbiased electron-density map of good quality to be generated. This study demonstrates the strength of S-¬SAD for phasing using accurate highly redundant data at low resolution.

Subject Areas: Biology and Bio-materials

Facility: X06SA at SLS

Added On: 16/04/2010 15:00

Discipline Tags:

Structural biology Life Sciences & Biotech

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