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The use of polyoxometalates in protein crystallography – An attempt to widen a well-known bottleneck

DOI: 10.1016/j.ccr.2015.03.018 DOI Help

Authors: Aleksandar Bijelic (University of Vienna) , Annette Rompel (University of Vienna)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Coordination Chemistry Reviews , VOL 299 , PAGES 22 - 38

State: Published (Approved)
Published: September 2015
Diamond Proposal Number(s): 8476

Open Access Open Access

Abstract: Polyoxometalates (POMs) are discrete polynuclear metal-oxo anions with a fascinating variety of structures and unique chemical and physical properties. Their application in various fields is well covered in the literature, however little information about their usage in protein crystallization is available. This review summarizes the impact of the vast class of POMs on the formation of protein crystals, a well-known (frustrating) bottleneck in macromolecular crystallography, with the associated structure elucidation and a particular emphasis focused on POM's potential as a powerful crystallization additive for future research. The Protein Data Bank (PDB) was scanned for protein structures with incorporated POMs which were assigned a PDB ligand ID resulting in 30 PDB entries. These structures have been analyzed with regard to (i) the structure of POM itself in the immediate protein environment, (ii) the kind of interaction and position of the POM within the protein structure and (iii) the beneficial effects of POM on protein crystallography apparent so far.

Journal Keywords: Macromolecular crystallography; Protein crystal; Polyoxometalate; X-ray structure analysis; Crystallization additive; Electrostatic interactions

Subject Areas: Chemistry


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