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Imperfection and radiation damage in protein crystals studied with coherent radiation

DOI: 10.1107/S1600577515019700 DOI Help
PMID: 26698068 PMID Help

Authors: Colin Nave (Diamond Light Source) , Geoff Sutton (University of Oxford) , Gwyndaf Evans (Diamond Light Source) , Robin Owen (Diamond Light Source) , Christoph Rau (Diamond Light Source) , Ian Robinson (London Centre for Nanotechnology, University College London) , Dave Stuart (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 23 , PAGES 228 - 237

State: Published (Approved)
Published: January 2016

Open Access Open Access

Abstract: Fringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X-ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the submicrometre scale. In addition, these features can provide more accurate intensity measurements (e.g. by model-based profile fitting), detwinning (by distinguishing the various components), phasing (by exploiting sampling of the molecular transform) and refinement (by distinguishing regions with different unit-cell parameters). In order to exploit these potential benefits, the features due to coherent diffraction have to be recorded and any change due to radiation damage properly modelled. Initial results from recording coherent diffraction at cryotemperatures from polyhedrin crystals of approximately 2 mm in size are described. These measurements allowed information about the type of crystal imperfections to be obtained at the sub-micrometre level, together with the changes due to radiation damage

Journal Keywords: Coherent Diffraction; Crystal Perfection; Radiation Damage

Subject Areas: Technique Development, Biology and Bio-materials

Instruments: I24-Microfocus Macromolecular Crystallography

Imperfection and radiation damage in protein crystals studied.pdf