Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins

DOI: 10.1016/j.febslet.2010.04.015

Authors: Yo Sonoda (Imperial College London) , Alex Cameron (Imperial College London) , Simon Newstead (University of Oxford) , Hiroshi Omote (Okayama University) , Yoshinori Moriyama (Okayama University) , Michihiro Kasahara (Teikyo University) , So Iwata (Imperial College London; Japan Science and Technology Agency; Diamond Light Source) , David Drew (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Febs Letters , VOL 584 (12) , PAGES 2539 - 2547

State: Published (Approved)
Published: June 2010

Abstract: The rate at which X-ray structures of membrane proteins are solved is on a par with that of soluble proteins in the late 1970s. There are still many obstacles facing the membrane protein structural community. Recently, there have been several technical achievements in the field that have started to dramatically accelerate structural studies. Here, we summarize these so-called ‘tricks-of-the-trade’ and include case studies of several mammalian transporters.

Journal Keywords: Membrane protein; X-ray structure; Fluorescence-detection size-exclusion chromatography; Green Fluorescent Protein; Mammalian transporter

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Membrane Protein Laboratory (MPL)
Instruments: NONE-No attached Diamond beamline

Added On: 01/02/2016 14:08

Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags: