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Membrane's Eleven: heavy-atom derivatives of membrane-protein crystals
DOI:
10.1107/S0907444906023547
PMID:
16855303
Authors:
Preben
Morth
(University of Aarhus)
,
Thomas
Sorensen
(Diamond Light Source)
,
Poul
Nissen
(University of Aarhus)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Acta Crystallographica Section D Biological Crystallography
, VOL 62 (8)
, PAGES 877 - 882
State:
Published (Approved)
Published:
August 2006
Abstract: A database has been assembled of heavy-atom derivatives used in the structure determination of membrane proteins. The database can serve as a guide to the design of experiments in the search for heavy-atom derivatives of new membrane-protein crystals. The database pinpoints organomercurials, platinum(II) and trimethyllead compounds as being particularly useful. On the other hand, lanthanide and uranyl compounds are poorly represented, which may be a consequence of these compounds having aggressive effects in crystal-soaking procedures. Furthermore, the database highlights the variety of methods applied in the preparation of heavy-atom-derivatized crystals and in phasing. Cocrystallization can be further exploited. Phases have predominantly been obtained by SIRAS/MIRAS methods rather than SAD/MAD in recent structure determinations.
Journal Keywords: membrane proteins; heavy-atom derivatives.
Subject Areas:
Biology and Bio-materials
Technical Areas:
Added On:
02/02/2016 15:14
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