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Imaging and radiation effects of gold nanoparticles in tumour cells

DOI: 10.1038/srep19442 DOI Help

Authors: Harold N Mcquaid (Queen's University of Belfast) , Mark F. Muir (Camlin Technologies Ltd) , Laura E. Taggart (Queen's University of Belfast) , Stephen J Mcmahon (Queen's University, Belfast) , Jonathan A. Coulter , Wendy B. Hyland , Suneil Jain (Queen's University, Belfast) , Karl T. Butterworth (Queen's University, Belfast) , Giuseppe Schettino (National Physical Laboratory) , Kevin M. Prise (Queen's University, Belfast) , David G. Hirst (Queen's University, Belfast) , Stanley W Botchway (Science and Technology Facilities Council (STFC)) , Fred J Currell (Queen's University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 6

State: Published (Approved)
Published: January 2016

Open Access Open Access

Abstract: Gold nanoparticle radiosensitization represents a novel technique in enhancement of ionising radiation dose and its effect on biological systems. Variation between theoretical predictions and experimental measurement is significant enough that the mechanism leading to an increase in cell killing and DNA damage is still not clear. We present the first experimental results that take into account both the measured biodistribution of gold nanoparticles at the cellular level and the range of the product electrons responsible for energy deposition. Combining synchrotron-generated monoenergetic X-rays, intracellular gold particle imaging and DNA damage assays, has enabled a DNA damage model to be generated that includes the production of intermediate electrons. We can therefore show for the first time good agreement between the prediction of biological outcomes from both the Local Effect Model and a DNA damage model with experimentally observed cell killing and DNA damage induction via the combination of X-rays and GNPs. However, the requirement of two distinct models as indicated by this mechanistic study, one for short-term DNA damage and another for cell survival, indicates that, at least for nanoparticle enhancement, it is not safe to equate the lethal lesions invoked in the local effect model with DNA damage events.

Journal Keywords: Biological physics; Nanoscale biophysics; Radiotherapy; Targeted therapies

Diamond Keywords: Breast Cancer

Subject Areas: Biology and Bio-materials, Medicine

Instruments: B16-Test Beamline , I15-Extreme Conditions

Added On: 09/02/2016 12:04


Discipline Tags:

Non-Communicable Diseases Health & Wellbeing Cancer Materials Science Nanoscience/Nanotechnology Biophysics Life Sciences & Biotech

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