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Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography

DOI: 10.1038/srep14088 DOI Help
PMID: 26404036 PMID Help

Authors: Juliana Martins De S. E Silva (Technische Universität München) , Irene Zanette (Diamond Light Source) , Peter B. Noël (Technische Universität München) , Mateus B. Cardoso (Brazilian Synchrotron Light Laboratory) , Melanie A. Kimm (Technische Universität München) , Franz Pfeiffer (Technische Universität München)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 5

State: Published (Approved)
Published: September 2015

Open Access Open Access

Abstract: Low inherent contrast in soft tissues has been limiting the use of X-ray absorption micro-computed tomography (micro-CT) to access high-resolution structural information of animal organs. The staining agents used in micro-CT to improve the contrast fail in providing high-quality images of whole organs of animals due to diffusion problems of the staining agent into the sample. We demonstrate a staining protocol that incorporates a biochemical conditioning step prior to exposure to the staining agent that succeeds in overcoming the diffusion problems, thus quickly providing high-quality micro-CT images of whole organs of mammals. Besides of yielding non-distorted three-dimensional information at the same spatial resolution accessible in histological sections, micro-CT images of whole organs stained by our method enable easy screening of slices along any direction of the volume thus demonstrating new possibilities of structural analysis in biomedical science.

Journal Keywords: Biological physics; Biophysics

Subject Areas: Biology and Bio-materials

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Added On: 09/02/2016 14:27

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