Cryo-soft X-ray tomography: a journey into the world of the native-state cell

DOI: 10.1007/s00709-013-0583-y

Authors: Raffaella Carzaniga (Cancer Research UK) , Marie-Charlotte Domart (Cancer Research UK) , Lucy M. Collinson (Cancer Research UK) , Elizabeth Duke (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Protoplasma , VOL 251 (2) , PAGES 449 - 458

State: Published (Approved)
Published: March 2014

Abstract: One of the ultimate aims of imaging in biology is to achieve molecular localisation in the context of the structure of cells in their native state. Here, we review the current state of the art in cryo-soft X-ray tomography (cryo-SXT), which is the only imaging modality that can provide nanoscale 3D information from cryo-preserved, unstained, whole cells thicker than 1 μm. Correlative cryo-fluorescence and cryo-SXT adds functional information to structure, enabling studies of cellular events that cannot be captured using light, electron or X-ray microscopes alone.

Journal Keywords: Cryo-soft X-ray tomography; Cryo-fluorescence; Native state; Correlative; Cells

Subject Areas: Biology and Bio-materials


Technical Areas:

Added On: 09/02/2016 16:52

Documents:
Carzaniga2014_Article_Cryo-softX-rayTomographyAJourn.pdf

Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags: