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Molecular basis of ion permeability in a voltage-gated sodium channel

DOI: 10.15252/embj.201593285 DOI Help
PMID: 26873592 PMID Help

Authors: C. E. Naylor (Birkbeck College, Birkbeck College University of London) , C Bagneris (Birkbeck College, Birkbeck College University of London) , P. G. Decaen (Howard Hughes Medical Institute Boston Children's Hospital) , Altin Sula (Birkbeck College, Birkbeck College University of London) , A. Scaglione (Howard Hughes Medical Institute Boston Children's Hospital) , D. E. Clapham (Howard Hughes Medical Institute Boston Children's Hospital) , Bonnie Wallace (Birkbeck College, Birkbeck College University of London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Embo Journal , VOL 35 (4) , PAGES 371 - 457

State: Published (Approved)
Published: February 2016

Abstract: Voltage?gated sodium channels are essential for electrical signalling across cell membranes. They exhibit strong selectivities for sodium ions over other cations, enabling the finely tuned cascade of events associated with action potentials. This paper describes the ion permeability characteristics and the crystal structure of a prokaryotic sodium channel, showing for the first time the detailed locations of sodium ions in the selectivity filter of a sodium channel. Electrostatic calculations based on the structure are consistent with the relative cation permeability ratios (Na+ ? Li+ ? K+, Ca2+, Mg2+) measured for these channels. In an E178D selectivity filter mutant constructed to have altered ion selectivities, the sodium ion binding site nearest the extracellular side is missing. Unlike potassium ions in potassium channels, the sodium ions in these channels appear to be hydrated and are associated with side chains of the selectivity filter residues, rather than polypeptide backbones.

Journal Keywords: Crystal StructureElectrophysiology Ion PermeabilitySodium Channel

Subject Areas: Biology and Bio-materials


Instruments: I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography

Other Facilities: Soleil; ESRF

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