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Heterogeneity of Collagen VI Microfibrils: Structural Analysis of Non-collagenous Regions

DOI: 10.1074/jbc.M115.705160 DOI Help

Authors: Tobias Maaß (University of Cologne) , Christopher P Bayley (University of Manchester) , Matthias Mörgelin (Lund University) , Sandra Lettmann (University of Manchester) , Paolo Bonaldo (University of Padova) , Mats Paulsson (University of Cologne) , Clair Baldock (University of Manchester) , Raimund Wagener (University of Cologne)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Biological Chemistry

State: Published (Approved)
Published: January 2016

Abstract: Collagen VI, a collagen with uncharacteristically large N- and C-terminal non-collagenous regions, forms a distinct microfibrillar network in most connective tissues. It was long considered to consist of three genetically distinct α chains (α1, α2 and α3). Intracellularly heterotrimeric molecules associate to form dimers and tetramers, which are then secreted and assembled to microfibrils. The identification of three novel long collagen VI α chains α4, α5, and α6 led to the question if and how these may substitute for the long α3 chain in collagen VI assembly. Here we studied structural features of the novel long chains, and analyzed the assembly of these into tetramers and microfibrils. N- and C-terminal globular regions of collagen VI were recombinantly expressed and studied by SAXS. Ab initio models of the N-terminal globular regions of the α4, α5 and α6 chains showed a C-shaped structure similar to that found for the α3 chain. Single particle EM nanostructure of the N-terminal globular region of the α4 chain confirmed the C-shaped structure revealed by SAXS. Immuno EM of collagen VI extracted from tissue revealed that like the α3 chain the novel long chains assemble to homotetramers that are incorporated into mixed microfibrils. Moreover, SAXS models of the C-terminal globular regions of the α1, α2, α4, and α6 chains were generated. Interestingly, the α1, α2 and α4 C-terminal globular regions dimerize. These self-interactions may play a role in tetramer formation.

Journal Keywords: collagen electron microscopy; (EM); extracellular matrix; single particle analysis; small-angle X-ray scattering (SAXS); VWA domain; microfibrils

Subject Areas: Biology and Bio-materials

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: Petra III

Added On: 25/02/2016 14:55

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