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Direct mapping of 19 F in 19 FDG-6P in brain tissue at subcellular resolution using soft X-ray fluorescence

DOI: 10.1088/1742-6596/463/1/012003 DOI Help

Authors: C Poitry - Yamate (Ecole polytechnique Federale de Lausanne) , A Gianoncelli (ELETTRA) , G Kourousias (ELETTRA) , B Kaulich , M Lepore (Ecole Polytechnique Fédérale de Lausanne) , R Gruetter (Ecole Polytechnique Fédérale de Lausanne) , M Kiskinova (ELETTRA)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Physics: Conference Series , VOL 463 (1)

State: Published (Approved)
Published: October 2013

Open Access Open Access

Abstract: Low energy x-ray fluorescence (LEXRF) detection was optimized for imaging cerebral glucose metabolism by mapping the fluorine LEXRF signal of 19F in 19FDG, trapped as intracellular 19F-deoxyglucose-6-phosphate (19FDG-6P) at 1μm spatial resolution from 3μm thick brain slices. 19FDG metabolism was evaluated in brain structures closely resembling the general cerebral cytoarchitecture following formalin fixation of brain slices and their inclusion in an epon matrix. 2-dimensional distribution maps of 19FDG-6P were placed in a cytoarchitectural and morphological context by simultaneous LEXRF mapping of N and O, and scanning transmission x-ray (STXM) imaging. A disproportionately high uptake and metabolism of glucose was found in neuropil relative to intracellular domains of the cell body of hypothalamic neurons, showing directly that neurons, like glial cells, also metabolize glucose. As 19F-deoxyglucose-6P is structurally identical to 18F-deoxyglucose-6P, LEXRF of subcellular 19F provides a link to in vivo 18FDG PET, forming a novel basis for understanding the physiological mechanisms underlying the 18FDG PET image, and the contribution of neurons and glia to the PET signal.

Journal Keywords: Brain; Detection; Fluorescence; Fluorine 18; Fluorine 19; Glucose; In Vivo; Metabolism; Nerve Cells; Phosphates; Signals; Soft X Radiation; Spatial Resolution

Subject Areas: Physics


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