Interaction of magnetic nanoparticles with U87MG cells studied by synchrotron radiation X-ray fluorescence techniques

DOI: 10.1002/xrs.2475

Authors: A. Gianoncelli (ELETTRA Sincrotrone Trieste) , P. Marmorato (European Commission, Joint Research Centre, IHCP) , J. Ponti (European Commission, Joint Research Centre, IHCP) , L. Pascolo (IRCCS Burlo Garofolo) , B. Kaulich (ELETTRA Sincrotrone Trieste; Diamond Light Source; ELETTRA) , C. Uboldi (European Commission, Joint Research Centre, IHCP) , F. Rossi (European Commission, Joint Research Centre, IHCP) , D. Makovec (Jozef Stefan Institute) , M. Kiskinova (ELETTRA Sincrotrone Trieste,) , Giacomo Ceccone (European Commission, Joint Research Centre, IHCP)
Co-authored by industrial partner: No

Type: Conference Paper
Conference: European Conference on X-Ray Spectrometry (2012)
Peer Reviewed: No

State: Published (Approved)
Published: July 2013

Abstract: Synchrotron radiation (SR) X-ray microscopy combined with X-ray fluorescence (XRF) microspectroscopy provides unique information that have pushed the frontiers of biological research, particularly when investigating intracellular mechanisms. This work reports an SR-XRF microspectroscopy investigation on the distribution and the potential toxicity of Fe2O3 and CoFe2O4 nanoparticles (NPs) in U87MG glioblastoma-astrocytoma cells. The U87MG cells exposed to NPs concentrations ranging from 5 to 250 µg/ml for 24 h were analyzed in order to monitor both morphological and chemical changes. The SR-XRF maps complemented with XRM absorption and phase contrast images have revealed different intracellular distribution patterns for the two nanoparticles types allowing different mechanism of toxicity to be deduced

Subject Areas: Materials

Facility: TwinMic at ELETTRA

Added On: 02/03/2016 14:10

Discipline Tags:

Materials Science Nanoscience/Nanotechnology

Technical Tags: