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Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution

DOI: 10.1038/srep20112 DOI Help

Authors: Hanfei Yan (Brookhaven National Laboratory) , Evgeny Nazaretski (Brookhaven National Laboratory) , Kenneth Lauer (Brookhaven National Laboratory) , Xiaojing Huang (Brookhaven National Laboratory) , Ulrich Wagner (Diamond Light Source) , Christoph Rau (Diamond Light Source) , Mohammed Yusuf (University College London (UCL)) , Ian Robinson (RCaH, UCL) , Sebastian Kalbfleisch (Brookhaven National Laboratory) , Li Li (Brookhaven National Laboratory) , Nathalie Bouet (Brookhaven National Laboratory) , Juan Zhou (Brookhaven National Laboratory) , Ray Conley (Brookhaven National Laboratory) , Yong S. Chu (Brookhaven National Laboratory)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 6

State: Published (Approved)
Published: February 2016

Open Access Open Access

Abstract: We developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray’s superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.

Journal Keywords: Fluorescence imaging; Imaging techniques

Subject Areas: Physics, Energy

Instruments: I13-1-Coherence

Other Facilities: National Synchrotron Light Source