Substrate Selectivity Analyses of Factor Inhibiting Hypoxia-Inducible Factor

DOI: 10.1002/ange.201208046

Authors: Ming Yang (University of Oxford) , Adam P. Hardy (University of Oxford) , Rasheduzzaman Chowdhury (University of Oxford) , Nikita D. Loik (University of Oxford) , John S. Scotti (University of Oxford) , James S. O. Mccullagh (University of Oxford) , Timothy D. W. Claridge (University of Oxford) , Michael A. Mcdonough (University of Oxford) , Wei Ge (University of Oxford) , Christopher J. Schofield (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Angewandte Chemie , VOL 125 (6) , PAGES 1744 - 1748

State: Published (Approved)
Published: February 2013

Abstract: Substrate specificity: Biochemical and crystallographic analyses reveal the hypoxia-inducible factor hydroxylase (FIH) as being promiscuous with respect to the residues that it can hydroxylate in β-position, which in addition to Asn, Asp, and His include Leu and Ser residues. The Ser substrate is oxidized to an epimeric β-geminal diol product

Journal Keywords: Ankyrins; Binding; Biocatalysis; Catalytic; Hydroxylation; Hypoxia-Inducible; Magnetic; Molecular; Peptides; Spectrometry; Mass; Matrix-Assisted; Substrate Specificity

Subject Areas: Chemistry, Biology and Bio-materials


Instruments: I03-Macromolecular Crystallography