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Radiation damage and derivatization in macromolecular crystallography: a structure factor's perspective

DOI: 10.1107/S2059798315021555 DOI Help

Authors: Robin Owen (Diamond Light Source) , Darren Sherrell (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Structural Biology , VOL 72 (3) , PAGES 388 - 394

State: Published (Approved)
Published: March 2016

Open Access Open Access

Abstract: During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site-specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site-specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X-ray-induced transformations is compared with the changes expected from derivatization, and strategies for minimizing radiation damage are discussed.

Journal Keywords: Phasing; radiation damage; macromolecular crystallography.

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

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