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Phase behavior of lysozyme solutions in the liquid–liquid phase coexistence region at high hydrostatic pressures

DOI: 10.1039/C6CP01791F DOI Help

Authors: Julian Schulze (TU Dortmund) , Johannes Möller (ESRF) , Jonathan Weiner (TU Dortmund University) , Karin Julius (TU Dortmund University) , Nico König (TU Dortmund University) , Julia Nase (TU Dortmund University) , Michael Paulus (TU Dortmund University) , Metin Tolan (TU Dortmund University) , Roland Winter (TU Dortmund University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Physical Chemistry Chemical Physics

State: Published (Approved)
Published: May 2016

Abstract: We present results from small-angle X-ray scattering and turbidity measurements on the effect of high hydrostatic pressure on the phase behavior of dense lysozyme solutions in the liquid–liquid phase separation region, and characterize the underlying intermolecular protein–protein interactions as a function of temperature and pressure under charge-screening conditions (0.5 M NaCl). A reentrant liquid–liquid phase separation region is observed at elevated pressures, which may originate in the pressure dependence of the solvent-mediated protein–protein interaction. A temperature-pressure-concentration phase diagram was constructed for highly concentrated lysozyme solutions over a wide range of temperatures, pressures and protein concentrations including the critical region of the liquid–liquid miscibility gap.

Subject Areas: Chemistry, Biology and Bio-materials

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: ESRF - ID02

Added On: 12/05/2016 18:32

Discipline Tags:

Physical Chemistry Biochemistry Chemistry Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)